Tag

Part:BBa_K3612015:Design

Designed by: Jesus Durand, Matias Rojas, Diego Benites, Maria Castromonte   Group: iGEM20_UPCH_Peru   (2020-10-23)

Signal Peptide (PhDsbA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence optimized for Pseudoalteromonas nigrifaciens.


Source

The EMBL Databank accession number for PhdsbA locus is AJ634705.

References

(1) Giuliani M, Parrilli E, Sannino F, Apuzzo G, Marino G, Tutino ML. Soluble recombinant protein production in Pseudoalteromonas haloplanktis TAC125. Methods Mol Biol. 2015;1258:243–57.

(2) Madonna S, Papa R, Birolo L, Autore F, Doti N, Marino G, et al. The thiol-disulfide oxidoreductase system in the cold-adapted bacterium Pseudoalteromonas haloplanktis TAC 125: Discovery of a novel disulfide oxidoreductase enzyme. Extremophiles. 2006 Feb 23;10(1):41–51.